FHV FCV Test Feline Calicivirus Herpesvirus Ag Combo Rapid Test

Introduction

FHV FCV Test Feline Calicivirus Herpesvirus Ag Combo Rapid test is a lateral flow immunochromatographic assay for the qualitative detection of feline calicivirus antigen (FCV Ag) in cat eye, nasal and anal secretions or serum and plasma specimens. ) and feline herpesvirus type 1 antigen (FHV Ag). Sensitivity:97.00% Specificity:100.00% Accuracy:98.50%

Benefits

1. One-step whole blood, serum and plasma testing.

2. Simple operation, non-professional on-site inspection.

3. The detection process is fast, and the result will be displayed in about 15 minutes.

4. The test time is less than or equal to 10 seconds, and it can detect 270 samples per hour at the fastest.

5. The detection equipment is compact, easy to carry, mobile terminal operation, cloud platform quality control.

6. The detection sensitivity is high, comparable to that of enzyme-labeled kits.

Components

• Test devices

• Droppers

• Single Buffer

• IFU

Principle

FHV FCV Test Feline Calicivirus Herpesvirus Ag Combo Rapid test is based on sandwich lateral flow immunochromatography. The test device has a test window for viewing test runs and reading results. Before detection, the test window has an invisible T (test) area and C (control) area. When the processed sample is applied to the sample well on the device, the liquid will flow laterally across the surface of the test strip and react with the pre-coated monoclonal antibody. A t-line is visible if FCV antigen or FHV antigen is present in the specimen. Line C should always appear after applying an example, which indicates a valid result. In this way, the device can accurately indicate the presence of FCV antigen or FHV antigen in the specimen

Other Items

Test Procedure

-Collect dog’s ocular, nasal or anus secretions with the cotton Swab and make the Swab wet sufficiently.
-Insert the Swab into the provided assay buffer tube. Agitates it to get efficient sample extraction. 
-If using serum or plasma specimen, use the dropper to collect the specimen. 

-Place 3 drops of serum or plasma into the assay buffer tube for dilution. The diluted sample is used as sample extraction. 
-Take out the test card from the foil pouch and place it horizontally.
-Suck the treated sample extraction from the assay buffer tube and place 3 drops into each sample hole “S” of the test card. 
-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

INTERPRETATION OF ASSAY RESULT

- Positive (+): Whether the T line is clear or fuzzy, the "C" line and the region "T" line are present.

- Negative (-): Shows only the sharp C line. No T line.

- Invalid: no color lines in zone C. Whether the T line appears or not.

Precaution

-All reagents must be tested at room temperature.

-Do not take the test tape out of the bag before use.

-Do not use the test after the expiration date.

-The components in this kit have been used as a standard batch processing unit for quality control testing. Do not mix parts of different batch numbers.

-All specimens are potentially infected. Must be treated strictly in accordance with the local country's rules and regulations.

Limitation 

Canivet cdv-cav-cvi-cpiv antigen combination test is only used for in vitro veterinary diagnosis. All results should be considered with other clinical data and can be discussed with the veterinarian. When a positive result is detected, it is recommended to use further confirmation methods such as RT-PCR.