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African swine fever virus CD2v protein ELISA antibody detection kit

African swine fever virus CD2v protein ELISA antibody detection kit can be used for the differential diagnosis of pigs infected with African swine fever virus wild strains and CD2v gene deletion strains by detecting African swine fever virus CD2v protein specific antibodies in pig serum.

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Introduction


HWTAi®African swine fever virus CD2v protein ELISA antibody detection kit can be used for the differential diagnosis of pigs infected with African swine fever virus wild strains and CD2v gene deletion strains by detecting African swine fever virus CD2v protein specific antibodies in pig serum.

Benefits

1. One-step whole blood, serum and plasma testing.

2. Simple operation, non-professional on-site inspection.

3. The detection process is fast, and the result will be displayed in about 15 minutes.

4. The test time is less than or equal to 10 seconds, and it can detect 270 samples per hour at the fastest.

5. The detection equipment is compact, easy to carry, mobile terminal operation, cloud platform quality control.

6. The detection sensitivity is high, comparable to that of enzyme-labeled kits.



Principle


African swine fever virus CD2v protein ELISA antibody detection kit adopts indirect ELISA method. The recombinant African swine fever virus CD2v protein was pre-coated on the wells of the microplate strip. In the experiment, the diluted serum to be tested is added, and after incubation, if the sample contains African swine fever virus CD2v specific antibody, it will bind to the antigen on the microtiter plate; unbound antibody and other antibodies will be removed after washing. After the composition, the enzyme conjugate is added to specifically bind to the antigen-antibody complex on the microtiter plate; the unbound enzyme conjugate is removed by washing; the substrate solution is added to the microwell plate, and the blue is formed by enzyme After the stop solution was added to stop the reaction, the absorbance OD value in the reaction well was measured with a microplate reader at a wavelength of 450 nm.




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