ToRCH Test kit

The ToRCH IgM Antibodies Combo Test is a rapid chromatographic immunoassay for the qualitative detection of IgM antibodies to Toxoplasma gondii (Toxo), Rubella virus (Rubella), Cytomegalovirus (CMV), and Herpes simplex virus 1/2 (HSV 1/2) in serum or plasma to aid in the diagnosis of ToRCH.

Specimen collection:

1. The ToRCH IgM Antibodies Combo Test can be performed used on Serum or Plasma.
2. To collect whole blood, serum or plasma specimens following regular clinical laboratory
procedures.
3. Separate serum or plasma from blood as soon as possible to avoid hemolysis. Use only clear
non-hemolyzed specimens.
4. Testing should be performed immediately after specimen collection. Do not leave the
specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8℃ for up to 3 days. For long term storage, specimens should be kept below -20℃.

5. Bring specimens to room temperature prior to testing. Frozen specimens must be completely thawed and mixed well prior to testing. Specimens should not be frozen and thawed repeatedly.

Operating  Steps

Allow the test, specimen, buffer and/or controls to reach room temperature 15-30℃ (59-86℉) prior
to testing.
1. Bring the pouch to room temperature before opening it. Remove the test device from the
sealed pouch and use it as soon as possible.
2. Place the test device on a clean and level surface.
3. Hold the dropper vertically and transfer 1 drop of specimen (approximately 10μl) to the
specimen well(S) of the test device, then add 2 drops of buffer (approximately 80μl) and start
the timer. See illustration below.
4. Wait for the colored line(s) to appear. Read results at 15 minutes. Do not interpret the result
after 20 minutes